Practical study on quality control of pathological HE staining
Pathological diagnosis is the most authoritative diagnostic method in modern medicine. Pathological HE staining is a widely used pathological diagnosis method. Its accuracy depends on the technical level of the pathologist on the one hand and the histopathological treatment and staining effect on the other hand. .
Guo Haiyan of Jilin Provincial Cancer Hospital recently studied the quality control practice of pathological HE staining. It was found that the quality of pathological HE staining was related to the technical level, operational experience and quality control methods of the operators. Every operation step needs to be done strictly. The article was published in the 24th issue of the China Medical Guide in 2015.
HE staining: also known as hematoxylin-eosin staining, in which hematoxylin is Hematoxylin, referred to as H, and eosin is Eosin, referred to as E, which is a staining method for observation and identification of apoptosis and cell necrosis by ordinary light microscopy. The method has wide application range, can color various components of tissue cells, is convenient for comprehensive observation of tissue structure, and is suitable for various fixed liquid fixing materials, and is not easy to fade after dyeing and can be preserved for a long time. After HE staining, the nucleus was stained blue-purple by hematoxylin and the cytoplasm was stained with eosin to pink.
HE dyeing precautions
1. The HE dyeing time should be shortened or prolonged according to the maturity of the dye and the room temperature. When the room temperature is high, the dyeing is promoted, and the dyeing time can be shorter. Otherwise, the time can be extended appropriately. When the room temperature is low in winter, it can be dyed in an incubator.
2. Alkaline water can also be used when bluening, but it may be rejected by eluant blue. If time is too late, it should be washed with running water to make the slice blue, but it should be noted that the water should not be too large. The slices are peeled off and the color is blue until at any time.
3. Differentiation is the key to the success of HE staining. If the differentiation is improper, it will lead to uneven dyeing, or deep or shallow, and the obtained sectioning dyeing effect is poor. If the staining is moderate, you can cancel this step.
4, Yihong is mainly stained with cytoplasm, and the coloration should be matched with the density of the nuclei stained nuclei. If the nuclei are thicker, the cytoplasm should be densely stained to obtain a sharp contrast. Conversely, if the nucleus is stained lightly, the cytoplasm should also be lightly stained. A few drops of glacial acetic acid can be added to the eosin alcohol solution to promote the cytoplasm to be easily colored, and it is not easy to fade when dehydrated by ethanol.
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