Expression of ELISA results
First, the result of qualitative determination: before measuring a batch of samples, first determine the absorption value of the ELISA control, those greater than this value are positive, and those less than this value are negative.
Second, the results of quantitative determination: determine the standard concentration of the antigen ELISA reaction optical density to draw a standard curve. The optical density of the ELISA reaction of the sample to be measured is measured to check the amount of antigen. In the labeled antigen competition method, the enzyme activity of the labeled enzyme is 100%. After adding the unlabeled antigen as a standard sample, the curve is drawn as a percentage of the decreased enzyme activity, and the amount of antigen to be tested is found from the curve. As for the quantification of antibodies, it is necessary to draw a competitive resistance curve. When making a standard curve, a blank control measurement needs to be performed to correct it. Or in the measurement, the control solution is used as the zero-point measurement point.
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