Application of fetal bovine serum

Fetal bovine serum function

1. Fetal bovine serum

Each quality index meets the requirements of the appendix of the third edition of the "2005 Pharmacopoeia of the People's Republic of China"

1. Appearance, light yellow and clear appearance, no hemolysis, no sticky liquid.

2. Protein content 3.5% ～ 5.0% (w / v)

3. The content of hemoglobin ≤ 0.02% (w / v)

4. Negative sterility test

5. Mycoplasma test is negative

6. Bacterial endotoxin ≤5 (EU / ml)

7. Bovine diarrhea virus negative

8. E. coli phage negative

9. Support cell proliferation (Sp2 / 0-Ag14) growth curve (inoculation concentration) maximum proliferation concentration ≥2.5 × 106 / ml

Cell doubling time≤15h

Cloning rate ≥85%

The main components of serum:

Serum is a very complex mixture formed by the removal of fibrin from plasma. Although most of its components are known, some of them are still unclear. The composition and content of serum often depend on the sex, age, and physiological conditions of the donor animal. It differs from nutritional conditions. Serum contains various plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc. These substances are physiologically balanced to promote cell growth or inhibit growth activity.

Fetal bovine serum should be taken from fetal calves delivered by caesarean section; new bovine serum should be taken from newborn cattle within 24 hours of birth; calf serum should be taken from calves born from 10 to 30 days.

Obviously, fetal calf serum is of the highest quality, because fetal calf has not yet contacted the outside world, and the serum contains antibodies, complements and other components that are harmful to the cells.

Function: Bovine serum is the natural medium with the largest dosage in cell culture. It is rich in nutrients necessary for cell growth. It is often used for in vitro culture of animal cells and has extremely important functions.

1. Provide hormones for maintaining the exponential growth of cells, no or few nutrients in the basic medium, and the main low molecular nutrients.

2. Provide binding proteins that can recognize vitamins, lipids, metals and other hormones, and can bind or modulate the vitality of the substances they bind.

3. In some cases, the binding protein can combine with toxic metals and pyrogens to play a role in detoxification.

4. It is the source of factors needed for cells to adhere to the wall and spread on the plastic medium.

5. It acts as a pH buffer.

6. Provide protease inhibitors to inactivate remaining trypsin during cell passage and protect cells from harm.