Experimental principle of human cytokeratin 13 (CK-13) ELISA kit
English name: Human cytokeratin 13, CK-13 ELISA
1. The level of human cytokeratin 13 (CK-13) ELISAKit in the specimen was determined by the double antibody sandwich method.
2. First coat the microplate with purified human cytokeratin 13 (CK-13) ELISAKit antibody to make a solid phase antibody.
3. Then add cytokeratin 13 (CK-13) ELISAKit to the microwells coated with mAb, and then combine with HRP-labeled cytokeratin 13 (CK-13) ELISAKit antibody to form antibody-antigen-enzyme-labeled antibody After thorough washing, the complex is added with substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with cytokeratin 13 (CK-13) ELISAKit in the sample.
5. Finally, use a microplate reader to measure the absorbance (OD value) at a wavelength of 450 nm, and calculate the concentration of human cytokeratin 13 (CK-13) ELISAKit in the sample through the standard curve
.
Objective: To determine the content of cytokeratin 13 (CK-13) ELISAKit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children
Bring your own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
Product Range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit
Abs Frame Bag Filter,Pre Filter Bag,Pocket Pre Filter,Filter Bag Abs Frame
Ningbo Ifilter Purification Equipment CO.,Ltd. , https://www.nbifiltor.com