China Education Equipment Purchasing Network News: Recently, the news about Clenbuterol has attracted the attention of the media. The editor of this website collected and sorted the detection method of Clenbuterol. Through analysis and comparison, it was found that the colloidal gold immunochromatography method, the public Operation is very popular.
Clenbuterol is a kind of animal medicine, which is very harmful to human health. Adding Clenbuterol to the feed can increase the amount of lean meat of the animal, reduce the use of feed, make the meat market early, and reduce the cost.
Advanced detection technology is one of the important means to ensure food safety. Clenbuterol was tested according to GB / T 5009.192-2003 Determination of Clenbuterol Residues in Animal Food. Common detection methods include: gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA), colloidal gold negotiable chromatography (Colloidal gold immunochromatography) and liquid chromatography -Mass spectrometry / mass spectrometry etc.
Gas chromatography-mass spectrometry can combine the high-efficiency and rapid separation effect of chromatography with the qualitative analysis of high-sensitivity mass spectrometry. It can perform qualitative and quantitative analysis on a variety of residues, and has a higher detection limit. High-performance liquid chromatography is used in conjunction with pre-column extraction, purification and post-column fluorescence derivatization reactions and mass spectrometry (MS) systems to easily automate the analysis process. It has the advantages of good specificity, strong selectivity, high detection accuracy, and low false positive rate. The disadvantage is that the sample processing time is long and detection. Compared with high performance liquid chromatography, gas chromatography-mass spectrometry has the advantages of higher detection sensitivity and lower false positive rate. The enzyme-linked immunosorbent assay has the characteristics of fast detection speed and high sensitivity, and is suitable for rapid qualitative detection on site. The operation of colloidal gold immunochromatography is simple and convenient, among which the citizens of Clenbuterol (Clenbuterol) rapid detection card can operate by themselves. The detection accuracy of liquid chromatography-mass spectrometry / mass spectrometry is high, but the process is cumbersome and difficult to operate, which requires expensive instruments and is subject to certain restrictions in practical applications.
The editor believes that among the above five detection methods, gas chromatography-mass spectrometry, high performance liquid chromatography, and liquid chromatography-mass spectrometry / mass spectrometry have high accuracy, strong selectivity, high detection sensitivity, and low false positive rate. Advantages, but these methods require expensive instruments, have high capital investment requirements for users, and require professional operation, and the operation process is cumbersome. It is suitable for use in research places such as laboratories and has practical applications. Due to the larger limitation, there are certain obstacles to the popularization of equipment. The enzyme-linked immunosorbent assay and colloidal gold immunochromatography are not as accurate as the above three methods, but they are easy to operate and have high sensitivity. They are suitable for rapid on-site qualitative detection, especially colloidal gold immunochromatography The citizens can operate by themselves, which has a high popularity and brings great convenience to our daily life. Different groups should choose different methods to detect Clenbuterol according to their actual situation and needs.
Related Links:
Brief introduction of Clenbuterol and its testing methods and testing instruments
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