CO2 incubator
Definition of carbon dioxide incubator: it is to simulate the formation of a cell / tissue growth environment in the body of the incubator, such as stable temperature (37 ° C), stable CO2 level (5%), constant pH (pH) Value: 7.2-7.4), a relatively high relative humidity (95%), a device for in vitro culture of cells / tissues.
Scope of application: Widely used in the cultivation of cells, tissue culture and some special microorganisms, commonly used in cell dynamics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, antigen research and Production and cultivation of hybridoma cells to produce antibodies, in vitro fertilization (IVF), stem cells, tissue engineering, drug screening and other research fields.
Users have two basic requirements for the carbon dioxide incubator. One is that the carbon dioxide incubator can provide the most accurate and stable control of temperature, carbon dioxide concentration and humidity, so as to facilitate the progress of its research work; the second is that the carbon dioxide incubator can Effectively prevent microbial contamination in the incubator, and can regularly eliminate pollution to protect research results and prevent sample loss.
1. Micro-processing control system
The micro-processing control system is an operating system that maintains the steady state of temperature, humidity and CO2 concentration in the incubator. The use of a micro-processing control system and various other functional accessories (such as high and low temperature automatic adjustment and alarm devices, CO2 alarm devices, password protection settings, etc.) makes the operation and control of carbon dioxide incubators very simple. Such as: LEEC's PID microprocessor touch screen control system, which can strictly control the gas concentration and reduce its loss to a very low level, to ensure that the culture environment is constant and that the temperature in the box is accurate during long-term cultivation LCD display, graphical process monitoring, intervention event recording, etc. In addition, the alarm system is also indispensable, it can let you know the situation of the incubator in time and react to it, thereby minimizing the loss and ensuring the continuity of the experiment. Some incubators have an audible / optical alarm device. When the temperature changes by ± 0.5 ° C, or when the CO2 concentration changes by ± 5%, an automatic alarm will be given; some have an alarm display function for abnormal CO2 concentration; some have an alarm function for low voltage and power failure. These devices are designed for the convenience of users to reduce the tedious experiment process.
2. Classification
According to the heating method, it is divided into air-jacket heating and water-jacket heating. Both heating systems are accurate and reliable, and they have their own advantages and disadvantages. Water jacket heating is to maintain a constant temperature by surrounding the inner box with an independent water jacket layer. Its advantages: water is a good thermal insulation substance. When a power failure occurs, the water jacket system can Maintaining the temperature accuracy and stability in the incubator for a relatively long time is beneficial to users who are not very stable in the experimental environment (such as limited useful electricity or frequent power outages). Air-jacket heating directly heats the inner box through heaters located in the gas jacket layer of the box, also known as six-sided direct heating. Compared with the water jacket type, the air jacket type has the characteristics of faster heating and faster temperature recovery than the water jacket type incubator, which is particularly beneficial for short-term cultivation and cultivation that requires frequent opening and closing of the door of the box. In addition, for users, the air jacket design is simpler than the water jacket type (the water jacket type needs to add water, empty and clean the water tank, and constantly monitor the operation of the water tank, as well as potential pollution risks).
3. CO2 concentration control
Infrared sensor (IR) or thermal conductivity sensor (TC) for measurement. Both sensors have advantages and disadvantages. The working principle of the thermal conductivity sensor to monitor the concentration of CO2 is based on the continuous measurement of the thermal conductivity of the air in the cavity. The low thermal conductivity of the input CO2 gas will cause the thermal conductivity of the air in the cavity to change, which will produce a direct Proportional electrical signal. One disadvantage of the TC control system is that changes in the temperature and relative humidity in the cabinet will affect the accuracy of the sensor. When the box door is opened frequently, not only the CO2 concentration, but also the temperature and relative humidity will fluctuate greatly, thus affecting the accuracy of the TC sensor. When precise culture conditions are required and the doors of the incubator are frequently opened, this control system is not very suitable. Infrared sensor (IR) It uses an optical sensor to detect CO2 levels. The IR system includes an infrared emitter and a sensor. When the CO2 in the box absorbs part of the infrared light emitted by the transmitter, the sensor can detect the amount of infrared reduction, and the amount of infrared light absorbed corresponds to the level of CO2 in the box. , So that the concentration of CO2 in the box can be obtained. Because the IR system determines the CO2 concentration in the box through the reduction of infrared rays, and the particles in the box can reflect or partially absorb infrared rays, making the IR system more sensitive to the amount of particles in the box, the IR sensor is applied to the air inlet with a HEPA efficient air filter The incubator is more suitable.
4. Relative humidity
Humidity in the box is a very important factor for cultivation, but it is often overlooked. Maintain a sufficient humidity level and a fast enough humidity recovery rate (such as after opening and closing the door) to ensure that the culture will not fail due to excessive drying. At present, most carbon dioxide incubators generate moisture through the evaporation of the humidifying plate (the relative humidity level generated can reach about 95%, but the humidity recovery rate is very slow after the door is opened). Choose an incubator with a large humidity evaporation area as much as possible, because the larger the humidity evaporation area, the easier it is to reach the maximum relative saturation humidity and the shorter the humidity recovery time after opening and closing the door.
V. Control of pollutants
Pollution is a major factor leading to the failure of cell culture. The manufacturers of carbon dioxide incubators have designed a variety of different devices to reduce and prevent the occurrence of pollution. The main way is to minimize the areas and surfaces where microorganisms can grow and combine Automatic decontamination device to effectively prevent pollution. For example, in view of the fact that CO2 incubators are sometimes accompanied by mold growth during use, in order to ensure that the incubators are not contaminated and to ensure the biological cleanliness of the instrument box, some companies have developed and designed CO2 incubators with ultraviolet disinfection function; also A company designed HEPA high-efficiency filter can filter the air in the incubator, which can remove 99.97% of particles above 0.3um; in addition, the automatic high-temperature hot air sterilization device can make the temperature in the box reach high temperature (such as 200 ℃) to kill all pollution Microorganisms, even high temperature resistant microorganisms such as spores, these devices are more secure for cell culture.
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