Dairy goats are not only an important source of milk, but also an important agricultural and economic role in developing countries. Understanding the genetic background of goat mammary glands is important for studying the regulatory mechanisms that control tissue function and milk composition synthesis. However, the lack of complete transcriptome information in goat mammary glands hinders the development of related work. To this end, the team led by Professor Luo Jun of Northwest A&F University conducted a comprehensive measurement and in-depth analysis of the transcriptome of breast tissue in dairy goats*, and the results were published in the recent Funct Integr Genomics.
Professor Luo's team collected tissue from four different stages of goat mammary gland development, and the transcriptome was determined by transcriptome denovo sequencing, which produced about 25 GB of sequence information (the transcriptome denovo sequencing project was completed by Lianchuan Biotechnology). Sequence splicing assembly acquired 51,361 Unigenes, and approximately 60.07% of Unigenes shared homology with proteins in the NR database. Further functional classification by KOG, GO, and KEGG revealed that Unigenes from goat mammary glands participate in a variety of biological processes and metabolic pathways, including lipid metabolism and lactose metabolism. Quantitative PCR revealed that genes encoding FABP3, FASN, SCD, PLIN2, whey protein (LALBA and BLG), and casein (CSN1S1, CSN1S2, CSN2 and CSN3) were compared in the non-lactating period at 100 days postpartum and postpartum 310. There is a significant high expression in the sky. In addition to their role in lipid and protein synthesis, high expression at 310 days postpartum may contribute to the renewal of breast cells during pregnancy.
The above study is the first comprehensive measurement and description of the goat mammary transcriptome, providing genomic resources for the future study of lactation in ruminants.
*H Shi, J Zhu, J Luo, W Cao, H Shi, D Yao, J Li, Y Sun, H Xu, K Yu, J Loor (2014) Genes regulating lipid and protein metabolism are highly expressed in mammary gland of lactating Finnish goats. Func. & Int. Genomics. ePub ahead of print doi: 10.1007/s10142-014-0420-1 [article]
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Lianchuan Bio-headquarters is located in LC Sciences, LLC. After years of rapid development, it has become the industry's leading multi-omics technical service provider. The company has a proprietary Paraflo® microfluidic custom chip platform with independent intellectual property rights, which can provide scientific users with high-throughput customized expression chip solutions from genome, transcriptome, to proteome. We are the first service provider in the world to provide microRNA expression profiling. As one of the first companies in China to provide high-throughput sequencing technology services, Lianchuan Bio has an experienced technical team and a first-class bioinformatics expert group to provide researchers with multi-study sequencing services and deep personalized data analysis solutions. Program. In order to meet the researcher's cross-group high-throughput research needs, Lianchuan Bio has introduced proteomic quantitative analysis into the technical service line to provide users with a full-scale solution that runs through the central rules. Over the years, Lianchuan Bio has been working closely with domestic research teams for a long-term and close cooperation to help researchers discover scientific discoveries in biology, medicine, and agriculture and forestry. Global research users have published more than 600 high-level research papers using Lianchuan Bio's high-quality research services and high-quality omics experimental data, including 17 papers published in the top journals of Cell, Nature, and Science.
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