Human leukotriene B4 (LTB4) ELISA kit is only for research use. The LTB4 kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards for known LTB4 concentrations, Samples of unknown concentration were added to the microwell microplate for detection. LTB4 and biotinylated antibodies were first incubated simultaneously. After washing, avidin-labeled HRP was added. After incubation and washing, the unbound enzyme conjugate is removed, then the substrates A, B, and the enzyme conjugate are added simultaneously. Produce color. The depth of the color is proportional to the concentration of LTB4 in the sample. Kit Composition Name 96-well configuration 48-well configuration Remarks Microporous microplates 12 wells × 8 strips 12 wells × 4 no standards (1000 ng/L) 0.5 mL 0.5 mL Diluted standard dilutions 6 mL 3 mL according to the instructions Instructions for dilution of the sample dilution 6mL 3mL According to the instructions for dilution detection antibody-HRP 6mL 3mL without 20 × wash buffer 20mL 20mL according to the instructions to dilute the substrate A 6mL 3mL without substrate B 6mL 3mL no stop solution 6mL 3mL no seal film 2 sheets, 2 sheets without instructions, 1 part, 1 piece, no self-sealing bag, 1 piece, no note: Standards are diluted with standard dilutions to: 1000, 500, 250, 125, 62.5, 0 ng/L. Microplate reader (450nm) 2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL3. 37 °C incubator results to judge the standard curve: in the Excel worksheet, The concentration of the standard product is plotted on the abscissa, and the OD value is plotted as the ordinate. The linear regression curve of the standard is drawn, and the concentration values ​​of each sample are calculated according to the curve equation. The company's elisa kit can provide free testing services, customers can provide their own sample samples, and provide the raw data required by customers within seven working days. If you need more specific instructions, please contact our online customer service (QQ, telephone). The test kits sold by Beijing Jiehui Bogao Biotechnology Co., Ltd. are: Snow Leopard, Panda, Golden Gopher, Giraffe, Rat, Mouse, Human Potatoes, lentils, peas, parsley and Other animal and plant shipments are stable!
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