**Purpose of Use:**
This kit is designed for the quantitative determination of fumonisin (FB) residues in a variety of sample types, including animal feed, fish, shrimp, and meat tissues such as chicken, beef, and pork. It is also suitable for testing eggs, honey, milk, serum, and urine samples. The method provides a reliable and efficient way to monitor FB contamination in food and feed products, ensuring safety and compliance with regulatory standards.
**Principle of the Experiment:**
The assay is based on a direct competitive ELISA technique. Fumonisin (FB)-conjugated antigens are pre-coated onto microtiter plates. After adding the standard solutions or sample extracts, free fumonisin molecules in the samples compete with the pre-coated FB-conjugated antigens for binding sites on the anti-fumonisin (FB) antibody. A labeled abzyme (antibody conjugate) is then introduced, which binds to the antigen-antibody complex. Following incubation, the TMB substrate is added, causing a color change from blue to yellow upon the addition of the stop solution. The absorbance is measured at 450 nm using a microplate reader. The intensity of the color is inversely proportional to the concentration of fumonisin in the sample. By comparing the results with a standard curve, the exact concentration of fumonisin can be accurately determined. This method is sensitive, specific, and ideal for routine analysis in laboratories and quality control settings.
430 Stainless Steel Press Trash Bin
This home kitchen press trash can could easily open or close by hand press. It is designed with sealed lid which is different with those traditional top open trash bins. What's more, the stainless steel garbage bin press type is with high strength and high quality anti-collision plastic inner bucket to help you collect or take out the trash effortlessly.
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