1. To ensure the accuracy of the pipette, the error cannot exceed 2%. Can be determined with water and electronic balance. But it is best to have a professional to correct it.
2. To be equipped with a 20ul, 50ul, 100ul, 1000ul and a volley gun. After sucking up different liquids, replace the pipette tip. Even when drawing standard products.
3. The reagent kit should be taken out of the refrigerator 1 hour before the experiment, so that all reagents are returned to room temperature to make the results more stable.
4. Keep the substrate protected from light during the experiment.
5. When using a gun to suck up the liquid, the speed should not be too fast, so as not to generate bubbles and make the sucking amount inaccurate.
6. When aspirating liquid, use a gun with a range close to the required amount to aspirate to reduce errors.
7. When adding liquid to the enzyme-labeled hole, avoid the contact between the tip and the liquid in the hole, so that the droplet on the tip will contact the hole wall, and the droplet will flow down naturally.
8. After all the liquid is added, the enzyme plate can be gently shaken in parallel on the table for 30 seconds to mix the liquid. You can also use the shaking function of the microplate reader.
9. When warm bathing, seal the enzyme label plate with self-adhesive or adhesive tape to prevent the evaporation of water.
10. When washing the plates, each time the washing liquid is added, it should be allowed to stand for 1 minute to make the cleaning more thorough. When there is no plate washer, after pouring the liquid, the enzyme plate should be patted dry on the newspaper or tissue paper.
11. When the washing solution is not enough, you can use distilled water to prepare phosphate buffer solution of pH7.4, 0.02M, and add 0.1% Tween 20 as the washing solution. Long-term storage is possible after adding 1/1000 sodium azide.
12. The substrate is light sensitive and should be prepared immediately before use.
13. Before testing, turn on the microplate reader and make it stable for more than 10 minutes.
14. The substrate has certain toxicity, and the termination liquid is corrosive to the skin, so contact should be avoided as much as possible.
15. The sample to be tested should be clarified, otherwise it will affect the result.
16. Warm bath time should comply with the provisions of the kit.
17. Double-hole experiments should be done as much as possible to ensure the accuracy of the data.
18. Samples with doubts about the results should be confirmed by other methods
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Description of Kraft Paper Bag form Sunshine Packaging
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Kraft Paper Bag
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