Nano anti-counterfeiting glue

Nano anti-counterfeiting glue. Adjust the calf thymus DNA concentration to 600 ng/μl, agarose gel electrophoresis to detect the concentration of anti-counterfeit DNA, take 18 μl of calf thymus DNA, add 2 μl anti-counterfeit DNA, followed by serial dilution, add 0.1 g glue. The calf thymus DNA concentration in the glue was 100 μl/μl, and the concentration of anti-counterfeit DNA was decreased by 10 times. Fine iron wire was stirred and mixed, and 2 μl of the ultrapure water was concentrated in 100 μl and vortexed. Take 2 μl as template and 20 μl PCR reaction system. The highest detection limit is 10-11 g/μl. The glue was evenly spread on the paper, dried naturally, cut with a sharp blade 10mm2, placed in 20μl reaction system detection, the maximum detection limit of 10-11g/μl glue. Use a pin to draw the coated paper, add 50μl ultrapure water, and soak at 65°C for 100min. After centrifugation, take the supernatant as a template. The PCR reaction shows that the highest detection limit is also 10-11g/clear 1 glue.