The level of Chinese medicine production in China is very different from the world's advanced natural medicine extraction level, which has affected the status of Chinese patent medicine products in the world pharmaceutical market. China has joined the WTO. If domestic Chinese medicine and natural product manufacturers want to win the initiative in competition, they must adopt advanced extraction and separation technology to adapt the production and sales of Chinese proprietary medicine products to the long history of Chinese medicine. Therefore, the state has adopted extraction and separation technology as one of the key technologies to be developed in the next five to ten years.
Many effective chemical components in traditional Chinese medicine are intracellular components. When extracting, it is necessary to break the cell wall or cell membrane, and the existing methods are difficult to achieve a good destructive effect, thus affecting the extraction results. Due to its unique extraction mechanism and ideal extraction effect, circulating ultrasonic extraction has shown obvious advantages in the extraction of effective chemical components of traditional Chinese medicine. Therefore, it is the most promising application of modern high-tech extraction and separation technology in the extraction of chemical components of traditional Chinese medicine. High technology.
At the same time, the integrated automation complete production line developed by Beijing Hongxianglong Biotechnology Co., Ltd. uses computer control throughout the production process to avoid the unstable product quality caused by human factors. Independent innovation and technology integration, and the improvement of production automation control level, can greatly improve the level of Chinese traditional medicine extraction technology and products, enhance the competitiveness in the international market, and transform resource advantages into economic benefits.
The effective ingredients of traditional Chinese medicine include polysaccharides, flavonoids, terpenoids, steroids, alkaloids and phenolic components. The extraction methods are different: when the polysaccharides in traditional Chinese medicines are extracted, they are extracted by boiling water or alcohol reflux. The crude extract is degreased with petroleum ether and finally separated by precipitation; the extraction of flavonoids is mainly carried out by heating or cold soaking with ethanol; the flavonoids are extracted by alkali extraction acid precipitation; the extraction of alkaloids is generally According to different target products, organic solvent, acidic, neutral or weakly alkaline aqueous solution is used for extraction, and volatile components are steam distilled; hydrazine compounds are generally extracted by solvent reflux. The first condition for extracting natural ingredients from traditional Chinese medicines is that the extracts can enter the extraction medium quickly and efficiently. Since the active ingredients of traditional Chinese medicines are mostly intracellular products, it is generally necessary to destroy the raw material cell walls during the extraction process. The circulating ultrasonic extraction technology and device have been successfully used for the extraction of natural products from hundreds of Chinese herbal medicine raw materials. Some of the results are as follows:
(1) Terpenoids, lactones
Artemisinin is generally extracted at a temperature below 50 ° C with petroleum ether, gasoline cold dipping or stirring. The extraction rate is about 60%, and the extraction time is 24-48 hours. Artemisinin was extracted with HF-2B, and the effects of fluid circulation flow, ultrasonic time, ultrasonic intensity, solid/liquid ratio, extraction temperature and agitation speed on the extraction rate of Artemisia annua L. and petroleum ether loss were studied. Under the experimental conditions, the extraction rate of artemisinin was over 90%, the recovery rate of artemisinin was increased by more than 25% compared with the conventional extraction method, the extraction time was shortened to 20 minutes, and the recovery rate of petroleum ether was 90%, which was significantly improved compared with the conventional extraction method. .
(2) Flavonoids
In the extraction of flavonoids from Ginkgo biloba, the extraction rate of conventional boiled for 4 hours was 56.5%; at room temperature, extraction with HF-2B for 10 min, the extraction rate was 82.6%, extraction was 30 min, the extraction rate was 88.3%; at 40 ° C, HF-50G was used. After extraction for 30 min, the extraction rate was 91.6%.
When the propolis flavonoids were extracted, the extraction was carried out for 48 hours, and the extraction rate was 36%. After extraction with HF-2B for 30 minutes at room temperature, the extraction rate was 41.8%.
At 30 °C, extracting Epimedium with 50% ethanol HF-2B for 30 minutes, the extraction rate of flavonoids was 87%.
When flavonoids were extracted with HF-20B for 60 min, the total flavonoid yield was 11.5% in 80% ethanol and 8.8% in 85% ethanol.
(3) Alkaloids
When the betaine was extracted from Cistanche deserticola with water, extraction with HF-2B for 20 minutes was equivalent to the extraction of betaine for 24 hours with conventional leaching and reflux for 6 hours at 100 °C.
Using HF-2B as the extractant, extract the total alkaloids from the sand oysters, extract them by ultrasonic extraction at room temperature for 30 minutes, extract twice, and the extraction rate is over 85%. The three effects are better; the extraction is 4-6 than normal temperature. Hours/times, the effect of extracting three times is better, and the effect is higher than that of diafiltration for 24 hours; with chloroform as extractant, ultrasonic extraction is 7 times, which is better than conventional continuous extraction for one week.
(4) mites
When Cistanche tubulosa was extracted from Cistanche deserticola with methanol, extraction with HF-2B for 30 minutes was equivalent to the extraction rate of Cistanche deserticola with conventional leaching for 24 hours and 80 ° C for 5 hours.
At 30 °C, 50% ethanol HF-2B was used to extract Epimedium for 30 minutes, and the senile hoofing rate was 90%.
(5) Polysaccharides
When the polysaccharide was extracted from Cistanche deserticola, the polysaccharide extraction was carried out using HF-2B for 20 minutes, which was higher than the conventional extraction for 24 hours and 100 ° C reflux for 6 hours.
When sulphate polysaccharide was extracted from kelp by HF-2B, it was extracted at 30 ° C for 20 minutes to obtain the same sulfate ester polysaccharide extraction rate by boiling (100 ° C) for 5 hours.
(6) Tea polyphenols
Tea polyphenols and catechol were extracted from tea leaves, and the total amount of tea polyphenols and catechol was increased by more than 40% compared with 100 °C water extraction for 30 min. The traits of the obtained tea polyphenols and catechol samples were not improved. The difference does not change the structure of the components of tea polyphenols and catechol.
(7) Solvent extraction and extraction and purification in slurry extraction
The HF-2B was used to extract the sulfated polysaccharides from the solvent. It is hoped that the coupling and integration of low-micro content natural active ingredients extraction, filtration, concentration, crude extraction, separation and purification process can be directly obtained, and the purified products can be directly obtained. Dip. At room temperature, the ultrasonic action time is 30 minutes, the extractant concentration is 7%, etc., the polysaccharide extraction rate can reach more than 92%, the fucose content in the polysaccharide is ≥35%, and the pharmaceutical polysaccharide is obtained in one step (fucose content ≥30) %) requires that the cost is only about a quarter of the regular extraction.
The above test results show that the circulating ultrasonic extraction device has good amplification performance, and the same equipment selection may have a large difference in results.
Beijing Hongxianglong Biotechnology Co., Ltd. specializes in the R&D and production of a full range of circulating ultrasonic extraction devices and integrated automation production lines. Due to the good extraction effect of circulating ultrasonic extraction technology and devices, it has been used by hundreds of domestic units. Some of the units of use are as follows:
Beijing Tongrentang Technology Co., Ltd., Jilin Correction Pharmaceutical, Zhengda Youthbao Pharmaceutical Co., Ltd., Chengde Neck Rehabilitation Pharmaceutical Group Co., Ltd., Zhejiang Huayao Pharmaceutical Co., Ltd., Mudanjiang Youbo Pharmaceutical Co., Ltd., Taiwan Shenpu Pharmaceutical Co., Ltd. Co., Ltd., Guangdong Bangmin Pharmaceutical Factory Co., Ltd., Guangxi Changzhou Natural Medicine Development Co., Ltd., Huanggang Li Shizhen Pharmaceutical Technology Development Co., Ltd., Shenyang Kaiwotian Biotechnology Co., Ltd., Tianjin Jinhao Biotechnology Co., Ltd., Tianjin Shenghui Biotechnology Co., Ltd. Qingdao Haitai Biotechnology Co., Ltd., Heilongjiang Hart Bioengineering Co., Ltd., Zhuhai Shengyuan Biotechnology Co., Ltd., Xinjiang Dedao Biotechnology Co., Ltd., Gansu Yumen Tuoyu Technology Development Co., Ltd., Luoyang Green Source Bioengineering Limited. Research Center for Traditional Chinese Medicine Modernization of Peking University School of Pharmacy, Institute of Medicinal Plants of Chinese Academy of Medical Sciences, Kunming Institute of Chinese Medicine, Institute of Plant Protection of Fujian Academy of Agricultural Sciences, Natural Medicine Research Center of Jiangsu University, Kunming Institute of Medical Sciences, China Pharmaceutical University, Beijing University of Chinese Medicine, Guangzhou Hanfang Modern Chinese Medicine Research Center, Capital Medical University, Xi'an Medical College, Shenyang Pharmaceutical University, Heilongjiang Provincial Traditional Chinese Medicine Research Institute, Sichuan Academy of Chinese Medicine, Nanjing Shenghe Pharmaceutical New Drug Research Institute, Nanhai, Foshan City Chinese Medicine Biotechnology Industry Center of the Chinese Academy of Sciences.
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