Chen Xingfu et al. (1999) used 2.5% NaCl to extract the Streptococcus suis capsular polysaccharide antigen, selected 2% gelatin as the blocking solution, and 4% PEG2PBS as the clear diluent. ELISA was used to detect S. suis antibody. Strong specificity, easy and fast operation.
Huazhong Agricultural University Zhang Anding, Jin Meilin, et al. <2005) established the ELISA antibody detection method of Streptococcus suis type 2, according to the different capsular polysaccharide antigens of Streptococcus suis, the bacteria can be divided into 35 capsular serotypes to purify pigs Enzyme-labeled plate coated with capsular polysaccharide antigen of streptococcus type 2 was established, and an indirect ELISA detection method for detecting serum antibody of streptococcus type 2 was established, and the method was applied to the epidemiological investigation of swine serum in the summer of 2005 and 2005 in Sichuan Province. In the first half of the year, the epidemiological survey of swine sera in Hubei, Henan and Hunan provinces.
The ELISA method is simple, economical, and fast, and this method has been used in veterinary testing departments at all levels in China for many years and is suitable for popularization and application. At the same time, ELISA has high sensitivity and good specificity, and has certain practical value in the diagnosis, quarantine and epidemiological investigation of the disease. The grim situation of Streptococcus suis type 2 in China makes accurate detection very important. In order to meet the needs of quarantine at all levels of veterinary departments, they have assembled the Streptococcus suis type 2 ELISA antibody test kit according to the method of developing ELISA. Used for the diagnosis of Streptococcus suis type 2 and the monitoring of antibody levels.
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