Product Name: Collagenase Other Names: Collagenase/Collagen Hydrolase English Name: Collagenase
CAS number: 9001-12-1
Acting brand: sigma, Amresco, Spectrum
Supplier: Shanghai Hengyuan Biotechnology Co., Ltd. The chemical nature of collagenase is a kind of protein. Therefore, it is very sensitive to various factors such as temperature, pH and protein denaturation. It is highly susceptible to external conditions and changes itself. Conformation and nature.
Multiple types of collagenase Differential application Collagenase type I is mainly used for the separation of epithelial, lung, adipose and adrenal tissue cells. Collagenase type II is suitable for the separation of liver, bone, thyroid, heart and salivary gland tissue cells. Collagenase Type IV Collagenase IV contains at least 7 protease components with molecular weights ranging from 68-130 KD. It can digest a variety of tissues. Collagenase Type V Collagenase V contains at least 7 protease components with molecular weights ranging from 68-130 KD. It can be used for the separation of pancreatic islet tissue, separating connective tissue into individual cells. ·How to use collagenase 1. Cut the rinsed and trimmed tissue into small pieces of about 1~2mm. 2. Place the tissue block into the centrifuge tube and add 30~50 times the volume of collagenase solution. Tighten the lid.
3. Place the flask in a 37 ° C water bath or 37 ° C incubator, shake it every 3 minutes, if it can be placed in a 37 ° C constant temperature shock water bath. Digestion time is closely related to the type of tissue. For some tumor tissues or other dense connective tissues, the digestion time is 4~48h. For easily digestible tissues, it can be digested by 37°C for 15~45min. It can also be determined according to the specific conditions. . If the tissue block has been dispersed and the shape of the block is lost, it can be considered to have been fully digested by shaking into a cell mass or a single cell. After the epithelial tissue is digested by collagenase, because the epithelial cells are tolerant to this enzyme, some cell clusters may still be completely dispersed, but the agglomerated epithelial cells are more likely to grow than the dispersed single epithelial cells. Therefore, if there is no special need You don't have to deal with it any further.
4. Collect the digestive juice, centrifuge 1000r/min, 5min, remove the supernatant, centrifuge with Hanks liquid or serum-free medium for 1~2 times, remove the supernatant, add the culture solution to make the cell suspension, and inoculate the culture flask.
CAS number: 9001-12-1
Acting brand: sigma, Amresco, Spectrum
Supplier: Shanghai Hengyuan Biotechnology Co., Ltd. The chemical nature of collagenase is a kind of protein. Therefore, it is very sensitive to various factors such as temperature, pH and protein denaturation. It is highly susceptible to external conditions and changes itself. Conformation and nature.
Multiple types of collagenase Differential application Collagenase type I is mainly used for the separation of epithelial, lung, adipose and adrenal tissue cells. Collagenase type II is suitable for the separation of liver, bone, thyroid, heart and salivary gland tissue cells. Collagenase Type IV Collagenase IV contains at least 7 protease components with molecular weights ranging from 68-130 KD. It can digest a variety of tissues. Collagenase Type V Collagenase V contains at least 7 protease components with molecular weights ranging from 68-130 KD. It can be used for the separation of pancreatic islet tissue, separating connective tissue into individual cells. ·How to use collagenase 1. Cut the rinsed and trimmed tissue into small pieces of about 1~2mm. 2. Place the tissue block into the centrifuge tube and add 30~50 times the volume of collagenase solution. Tighten the lid.
3. Place the flask in a 37 ° C water bath or 37 ° C incubator, shake it every 3 minutes, if it can be placed in a 37 ° C constant temperature shock water bath. Digestion time is closely related to the type of tissue. For some tumor tissues or other dense connective tissues, the digestion time is 4~48h. For easily digestible tissues, it can be digested by 37°C for 15~45min. It can also be determined according to the specific conditions. . If the tissue block has been dispersed and the shape of the block is lost, it can be considered to have been fully digested by shaking into a cell mass or a single cell. After the epithelial tissue is digested by collagenase, because the epithelial cells are tolerant to this enzyme, some cell clusters may still be completely dispersed, but the agglomerated epithelial cells are more likely to grow than the dispersed single epithelial cells. Therefore, if there is no special need You don't have to deal with it any further.
4. Collect the digestive juice, centrifuge 1000r/min, 5min, remove the supernatant, centrifuge with Hanks liquid or serum-free medium for 1~2 times, remove the supernatant, add the culture solution to make the cell suspension, and inoculate the culture flask.
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